Journal: Frontiers in Molecular Neuroscience

Article Title: Ptbp2 re-expression rescues axon growth defects in Smn-deficient motoneurons

doi: 10.3389/fnmol.2024.1393779

Figure Lengend Snippet: Ptbp2 interacts with Smn in motoneurons. (A) Representative images of Smn-Ptbp2 PLA signal in cultured motoneurons at DIV 6 using anti-Smn and anti-Ptbp2 antibodies. Motoneuron morphology was visualized with anti-Tubb3 antibody. Scale bars, 10 and 5 μm (magnified areas). (B) Co-immunoprecipitation of Smn by anti-Ptbp2 from motoneuron lysate pre-treated with RNase A as indicated. (C) Representative images showing Smn immunofluorescence and Hnrnpr FISH in cultured motoneurons at DIV 6. Arrowheads indicate colocalization of Smn and Hnrnpr in granules. Scale bars, 10 and 2 μm (magnified areas). (D) Fluorescence intensity profiles of Smn and Hnrnpr at the location indicated by arrow 4 in (C) . (E) Representative images showing Ptbp2 and Smn immunofluorescence and Hnrnpr FISH in cultured motoneurons at DIV 6. Scale bars, 10 and 2 μm (magnified areas). (F) Fluorescence intensity profiles of Ptbp2, Smn and Hnrnpr at the location indicated by a line in (E) .

Article Snippet: Cells were fixed again for 10 min at room temperature in PLP, washed with DPBS, and stained with FITC-conjugated anti-Tubb3 antibody (130-131-158, Miltenyi Biotec).

Techniques: Cell Culture, Immunoprecipitation, Immunofluorescence, Fluorescence

Journal: Scientific Reports

Article Title: fMRI indicates cortical activation through TRPV1 modulation during acute gouty attacks

doi: 10.1038/s41598-019-48656-6

Figure Lengend Snippet: Cortical TRPV1 expression was enhanced by gouty arthritis. ( A ) ROI selection. ( B ) Cortical TRPV1 expression on the MSU-affected and control sides. ( C ) TRPV1 immunoreactivity intensity. ( D ) TRPV1 immunoreactivity coverage. ( E ) Immunoprecipitation of beta-tubulin with TRPV1 immunoblotting, which demonstrates a strong interaction between TRPV1 and neuronal filament proteins. The cropped images of the gels are used in the figure, and images of the full-length gels are presented in Supplementary Fig. S2. (***Indicates statistically significant difference with p < 0.001 after ANOVA). Scale bar = 50 µm.

Article Snippet: The primary antibodies used were: anti-TRPV1 (1:2000; PC420; Merck Millipore, Darmstadt, Germany); anti-COX-2 (1:1000; Thermo Fisher Scientific); β-tubulin (1:2000; MCA2703; Bio-Rad Laboratories); β-actin (1:4000; sc-47778; Santa Cruz Biotechnology).

Techniques: Expressing, Selection, Control, Immunoprecipitation, Western Blot